Publications

Urbanowski, J and R. C. Piper. (1999) The Iron Transporter Fth1p forms a complex with the Fet5 oxidase and resides on the vacuolar membrane. J. Biol. Chem. 274: 38061.

Mullock, BM, Smith, CW, Ihrke, G., Bright, NA, Lindsay,M, Parkinson, EJ, Brooks, DA, Parton, RG, James, DE, Luzio, JP, Piper RC. (2000) Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and is required for late endosome-lysosome fusion. Mol. Biol. Cell 11:137-153

Wade, N, Bryant, N.J., Connolly, L.M., Simpson, R.J., Luzio, J.P., Piper, R.C., James, D.E. (2001) Syntaxin 7 complexes with mVti1b, Syntaxin 6, VAMP8 and VAMP7 in B16 melanocytes. J. Biol. Chem. 276:19820-19827

Urbanowski, J.L. and R.C. Piper (2001) Ubiquitin sorts proteins into the intralumenal degradative compartment of the late-endosome/lysosome. Traffic 2:622-630
• Cover Art

Bilodeau, PS, Urbanowski, JL., Winistorfer, SC, and Piper, RC. (2002) The Vps27-Hse1 Protein Complex binds Ubiquitin and Mediates Protein Sorting at the Endosome. Nature Cell Biology 4:534 – 539
• Selected by Faculty-of-1000
• Highlight in Research Roundup: JCB (2002) 158:11

Viviane Poupon, Abigail Stewart, Sally Gray, Robert C. Piper, J. Paul Luzio (2003) The role of mVps18p in clustering, fusion and intracellular localization of late endocytic organelles Mol. Biol. Cell 14:4015-4027

Jihui Ren, Sebastian Yeo, Mahendra Wagle, Cong Liu, Regina Zahn, Hongyuan Yang, Robert C. Piper and Alan L. Munn (2003) Vps20p and Vfi1p Interact With Vps4p and Function in Multivesicular Body Sorting and Endosomal Transport in Saccharomyces cerevisiae J. Cell Sci. 116:3957-3970

Bilodeau, PS, Winistorfer, SC, William Kearney, Andrew Robertson and Piper, RC. (2003) Vps27 cooperates with ESCRT-I to sort ubiquitinated cargo at the endosome. J. Cell Biol. 163:237-243

Richardson, SCW, Winistorfer, SC, Poupon, V., Luzio, JP, and Piper, RC. (2004) The late Vps orthologs localize to early endosomes, the cytoskeleton and interact with Syntaxin 13. Mol. Biol. Cell 15:1197-1210

Scott, PM, Bilodeau, PS, Zdhankina, O, Winistorfer, SC, Allaman, M., Kearny, W, Robertson, AD, Piper, RC (2004) GGA proteins bind ubiquitin to facilitate sorting at the trans-Golgi network. Nature Cell Biology 6:252-259

• Selected by Faculty-of-1000
•News and Views in Nature Cell Biology (2004) 6:175-177

• Highlight in Nature Reviews Molecular Cell Biology (2004) 5:255

W. Michael Brown, Charles A. Yowell, Anna Hoard, Thomas A. Vander Jagt, Lucy A. Hunsaker, Lorraine M. Deck, Robert E. Royer, Robert C. Piper, John B. Dame, Michael T. Makler, and David L. Vander Jagt (2004) Comparative Structural Analysis and Kinetic Properties of Lactate Dehydrogenases from the Four Species of Human Malarial Parasite Biochemistry 43:6219 – 6229

Dove SK, Piper RC, McEwen RK, Yu JW, King MC, Hughes DC, Thuring J, Holmes AB, Cooke FT, Michell RH, Parker PJ, Lemmon MA. (2004) Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors. EMBO J. 5:1922-33.
• Selected by Faculty-of-1000

Pryor PR, Mullock BM, Bright NA, Lindsay MR, Gray SR, Richardson SC, Stewart A, James DE, Piper RC, Luzio JP. (2004) Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events. EMBO Rep. In Press

Bilodeau, PS, Winistorfer, SC, Allaman MM, Srendhran, K, Kearney, WR, Robertson, AD, Piper, RC (2004) The GAT domains of clathrin-associated GGA proteins have two ubiquitin-binding motifs. J. Biol. Chem. 279:54808-54816

Brown WM, Yowell CA, Hoard A, Vander Jagt TA, Hunsaker LA, Deck LM, Royer RE, Piper RC, Dame JB, Makler MT, Vander Jagt DL. (2004) Comparative structural analysis and kinetic properties of lactate dehydrogenases from the four species of human malarial parasites. Biochemistry. 43:6219-29.

Chu Y, Piper R, Richardson S, Watanabe Y, Patel P, Heistad DD. (2006) Endocytosis of extracellular

superoxide dismutase into endothelial cells: role of the heparin-binding domain.

Arterioscler Thromb Vasc Biol. 9:1985-1990

Ren, J., Kee, Y., Huibregtse, J., Piper, R.C. (2007) Hse1, a component of the yeast Hrs-STAM ubiquitin sorting complex, associates both ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies. Mol Biol Cell. Jan;18(1):324-35.

• Highlight in the ASCB publication InCyte 2/2007

Stern, KA, Visser Smit, GD, Place, TL, Winistorfer, S., Piper, RC, Nill, NL. (2007) EGF Receptor Fate is Controlled by Hrs Tyrosine Phosphorylation Sites that Regulate Hrs Degradation. Mol Cell Biol. 27:888-898

Wiradjaja F, Ooms LM, Tahirovic S, Kuhne E, Devenish RJ, Munn AL, Piper RC, Mayinger P, Mitchell CA. (2007) Inactivation of the phosphoinositide phosphatases Sac1p and Inp54p leads to accumulation of phosphatidylinositol 4,5-bisphosphate on vacuole membranes and vacuolar fusion defects.

J Biol Chem 282(22):16295-307.

Zhang Y, Zolov SN, Chow CY, Slutsky SG, Richardson SC, Piper RC, Yang B, Nau JJ, Westrick RJ, Morrison SJ, Meisler MH, Weisman LS. (2007) Loss of Vac14, a regulator of the signaling lipid phosphati dylinositol 3,5-bisphosphate, results in neurodegeneration in mice. Proc Natl Acad Sci U S A. 104(44):17518-17523.

Richardson SC, Wallom KL, Ferguson EL, Deacon SP, Davies MW, Powell AJ, Piper RC, Duncan R.

(2008) The use of fluorescence microscopy to define polymer localisation to the late endocytic compartments in cells that are targets for drug delivery. J Control Release. 127(1):1-11. Epub 08 Jan 4.

Ren J, Pashkova N, Winistorfer S, Piper RC. DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies. (2008) J Biol Chem. 83(31):21599-611

McCutchan TF, Piper RC, Makler MT. Use of malaria rapid diagnostic test to identify Plasmodium knowlesi infection. Emerg Infect Dis. 2008 Nov;14(11):1750-2.

Shields SB, Oestreich AJ, Winistorfer S, Nguyen D, Payne JA, Katzmann DJ, Piper R. ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting. J Cell Biol. 2009 Apr 20;185(2):213-24.
• Highlight in Faculty of 1000 Biology

• Highlight in accompanying mini-review in J. Cell Biol.

Kamadurai HB, Souphron J, Scott DC, Duda DM, Miller DJ, Daniel Stringer D, Piper RC, Schulman BA (2009) Insights into ubiquitin transfer cascades from a structure of a UbcH5B~Ubiquitin-HECTNEDD4L complex Mol Cell 36(6):1095-102.

Qui, L, Pashkova N., Walker JR, Winistorfer S, Allali-Hassani A, Akutsu, M, *Piper RC, *Dhe-Paganon S. (2010) Structure and Function of the Carboxy Terminal Armadillo Domain of Plaa/Doa1/Ufd3 Bound to the C-Terminus of p97/Cdc48 J. Biol. Chem. 285(1):365-72

Zhou R, Kabra R, Olson DR, Piper RC, Snyder PM. (2010) Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways. J Biol Chem. 2010 Jul 30. 285:30523-30530

Pashkova, NI, Gakhar, L, Winistorfer, SC, Yu, LP, Ramaswamy, S., Piper, RC (2010) WD40 repeat propellers define a ubiquitin binding domain that regulates turnover of F-box proteins
Molecular Cell. 12:433-443

• Highlight in Faculty of 1000 Biology

• Highlight in Cell Cycle (in process)

Stringer, D and Piper, RC. (2011) A Single Ub is sufficient for MVB sorting in the absence of ESCRT ubiquitination. J. Cell Biol. 192:229-242

• Highlight in Nature Reviews Molecular and Cellular Biology Epub 2/11/2011
• Highlight in Faculty of 100 Biology

Piper, RC., Buchanan, I., Choi, YH., Makler, MT. (2011) Opportunities for improving pLDH based diagnostic tests. Malaria Journal. 1:213.

MacDonald, C., Buchovich, NJ, Stringer, DK, Emr, SD, and Piper, RC (2012) Ubiquitinated Cargo is Essential for the ESCRT-dependent Formation of Multivesicular Body Intralumenal Vesicles. 13:331-338 EMBO Reports

MacDonald, C, Stinger, DK, Piper RC, (2012) Sna3 is an Rsp5 Adaptor Protein that Relies on Ubiquitination for Sorting into Multivesicular Bodies. 13:586-598 Traffic

Sinah N., Williams, CA, Piper, RC, Shields SB. (2012) A pair of Dual Promoter Vectors for High Throughput Cloning, Screening, and Expression of Eukaryotic and Prokaryotic Systems from a Single Plasmid. BMC Technology 12:54

Ver Heul, A.M, Fowler, A, Ramaswamy S. and Piper RC. (2013) Ubiquitin Regulates Caspase Recruitment domain mediated signaling by Nucleotide Binding oligomerization domain containing proteins NOD1 and NOD2. J. Biol. Chem. 288:6890-6902

Pashkova, N, Gakhar, L., Winistorfer, SW., Sunshine, A.B., Rich, M., Dunham, M.J., Yu, L., Piper, RC. (2013) The Yeast Alix homolog, Bro1, Functions as a Ubiquitin Receptor for Protein Sorting into Multivesicular Endosomes. Dev. Cell. 25:520-533

Sette P, Nagashima K., Piper RC., Bouamr, F. (2013) Ubiquitin conjugation to Gag is essential for ESCRT-mediated HIV-1 budding. Retrovirology 10:79

Kumadurai, HB., Qiu, Y., Deng A., Harrison JS., Macdonald C., Actis, M., Rodrigues, P., Miller DJ, Souphron J., Lewis SM., Kurinov, I., Hammel M., Piper, R., Kuhlman, B., Schulman, BA. (2013) Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3. eLIFE e00828

Peterson, TA, Yu, Liping*, Piper, RC. (2014) Backbone and Side-chain NMR Assignments for the C-terminal Domain of Mammalian Vps28 J. BioMol NMR Assignments. (DOI: 10.1007/s12104-013-9537-8)